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Nces (DCEXS), Universitat Pompeu Fabra, Carrer Doctor Aiguader 88, 08003 Barcelona, Spain Molecular and BI-0115 Autophagy Cellular Modeling Group, Heidelberg Institute for Theoretical Research (HITS), Schloss-Wolfsbrunnenweg 35, 69118 Heidelberg, Germany Genome Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany Biological Adaptation and Ageing (B2A), CNRS UMR 8256 INSERM ERL U1164, Institut de Biologie Paris-Seine (IBPS), Facultdes Sciences et d’Ing ierie (FSI), Sorbonne Universit 7 Quai St Bernard, CEDEX 05, 75252 Paris, France7 Facultat de Medicina y Ciencias de la Salud, Universitat de Barcelona, Carrer de Casanova 143, 08036 Barcelona, Spain Architecture et R ctivitde l’ARN, CNRS UPR 9002, Universitde Strasbourg, 2 All Conrad Roentgen, 67000 Strasbourg, France InstituciCatalana de Recerca i Estudis Avan ts (ICREA), Passeig de Llu Companys 23, 08010 Barcelona, Spain Expression G ique Microbienne, CNRS UMR 8261, Institut de Biologie Physico-Chimique (IBPC), Universitde Paris, 13 Rue Pierre et Marie Curie, 75005 Paris, France AIDS and Cancer Virus System, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21701, USA Biologie Int rative des Organismes Marins (BIOM), CNRS UMR 7232, Observatoire Oc nologique de Banyuls (OOB), Facultdes Sciences et d’Ing ierie (FSI), Sorbonne Universit 1 Avenue Pierre Fabre, 66650 Banyuls-sur-Mer, France Correspondence: [email protected] (S.L.); [email protected] (S.K.S.); [email protected] (G.M.) These authors contributed equally to this work.Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is definitely an open access short article distributed under the terms and conditions from the Inventive Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ four.0/).Abstract: A growing number of research indicate that mRNAs and long ncRNAs can impact protein populations by assembling dynamic ribonucleoprotein (RNP) granules. These phase-separated molecular `sponges’, stabilized by quinary (transient and weak) interactions, manage proteins involved in many biological functions. Retroviruses which include HIV-1 kind by self-assembly when their genomic RNA (gRNA) traps Gag and GagPol polyprotein precursors. Infectivity calls for extracellular budding with the particle followed by maturation, an ordered processing of 2400 Gag and 120 GagPol by the viral protease (PR). This results in a condensed gRNA-NCp7 nucleocapsid plus a CAp24-self-assembled capsid surrounding the RNP. The choreography by which all of those elements dynamically interact throughout virus maturation is amongst the missing milestones to completely depict the HIV life cycle. Here, we describe how HIV-1 has evolved a dynamic RNP granule with successive weak trong oderate quinary NC-gRNA networks through the sequential processing of your GagNC domain. We also reveal two palindromic RNA-binding triads on NC, KxxFxxQ and QxxFxxK, that present quinary NC-gRNA interactions. Consequently, the nucleocapsid complex seems correctly aggregated for capsid Hydroxyflutamide site reassembly and reverse transcription, mandatory processes for viral infectivity. We show that PR is sequestered within this RNP and drives its maturation/condensation inside minutes, this approach being most helpful in the end of budding. We anticipate su.

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