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D the p value 0.05, the gene was assumed to be differentially
D the p value 0.05, the gene was assumed to become differentially expressed. The samples were additional analyzed for gene expression difference evaluation, enrichment GYY4137 custom synthesis evaluation (GO enrichment analysis and KEGG pathway evaluation), and clustering analysis. The transcript sequence was lowered by splicing the reads around the comparison pairs. 3. Outcomes three.1. Improvement in Fruit Quality Two sweet orange varieties (LW and XC) responded drastically to Safranin Protocol Foliar application of EE-GRSP (Figures 1 and two). The fruits of LW with applied exogenous EE-GRSP recorded considerably greater Vitamin C content material than non-applied fruits; the fruits of XC with EE-Agriculture 2021, 11,three.three. Benefits Results 3.1. Improvement in Fruit Quality three.1. Improvement in Fruit QualityTwo sweet orange varieties (LW and XC) responded drastically to foliar application Two sweet orange varieties (LW and XC) responded drastically to foliar application of EE-GRSP (Figures 1 1 and two). The fruits of LW with applied exogenous EE-GRSP recorded of EE-GRSP (Figures and two). The fruits of LW with applied exogenous EE-GRSP recorded considerably greater Vitamin CC content material than non-applied fruits; the fruits of XC with EEsignificantly higher Vitamin content than non-applied fruits; the fruits of XC with EEGRSP displayed considerably larger titrable acidity andand loweringVitamin CC C content material GRSP displayed drastically larger titrable acidity lowering of Vitamin content GRSP displayed considerably higher titrable acidity and lowering of of Vitamincontent than of non-EE-GRSP (Figure 1). Foliar spraying of EE-GRSP drastically improved than thatthatnon-EE-GRSP (Figure 1). Foliar spraying of EE-GRSP substantially increased than that of of non-EE-GRSP (Figure 1). Foliar spraying of EE-GRSP substantially improved fruit sucrose, glucose, fructose concentrations when compared with the the non-EE-GRSP handle, fruit sucrose, glucose, andand fructose concentrations in comparison with non-EE-GRSP handle, fruit sucrose, glucose, and fructose concentrations in comparison to the non-EE-GRSP manage, regardless LW and XC varieties (Figure two). regardless of LWLW and XC varieties (Figure 2). irrespective of of and XC varieties (Figure 2).four ofFigure 1. Effects of exogenous effortlessly extractable glomalin-related soil protein (EE-GRSP) on Vitamin Figure 1.1. Effects of exogenous simply extractable glomalin-related soilLane Late Navel (LW) VitaFigure Effects of exogenous easily and titrable glomalin-related soil protein (EE-GRSP) on and Rohde C (mg/g), total soluble strong , extractable acidity of fruits of protein (EE-GRSP) on Vitamin Red (mg/g), total soluble solidsweetand titrable acidity of fruits five) followed by unique letters at C (mg/g), total soluble solid , orange. Information (means of fruits of Lane Late Navel (LW) and min C Valencia (XC) varieties of, and titrable acidity SD, n = of Lane Late Navel (LW) and Rohde Red Valencia (XC) varieties of sweet orange. Information (means SD, n = 5) followed by different Rohdebar indicate substantial variations (p 0.05) amongst(means SD, n = 5) followed by various the Red Valencia (XC) varieties of sweet orange. Information treatment options. letters atat the bar indicate significant differences (p 0.05) amongst therapies. letters the bar indicate substantial variations (p 0.05) among therapies.Figure two. Effects of exogenous, conveniently extractable glomalin-related soil protein (EE-GRSP) suFigure two.two. Effects of exogenous, simply extractable glomalin-related soil protein (EE-GRSP) on suFigure Effects of exoge.

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