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was observed that AAC Tenacious or AAC Innova shared their pedigrees with a minimum of 9 (out of each of the cultivars/genotypes with known pedigrees) various PHS-resistant cultivars/genotypes (AC Domain, Leader, Renan, HD2329, OS21, Opata, Cayuga, Danby and Rio Blanco) from six different countries (Canada, France, India, Japan, Mexico along with the USA) (Table two). AAC Tenacious and AAC Innova usually do not share their pedigrees with two resistant cultivars/genotypes, RSP (from China) and Zenkoujikomugi (from Japan) and susceptible landrace Chinese Spring (from China) (Table two).Discussion PHS is often a critical threat to wheat production in many growing areas, particularly exactly where late seasonal rainfall happens for the duration of harvest. In recent years, it has become more frequent as a result of uncertain weather conditions linked with climate alter [53]. Integrating PHS resistance in modern wheat cultivars is usually a significant breeding objective in quite a few nations including Australia, Canada, China, Japan and USA [53]. Seed dormancy is deemed the dominant element in controlling PHS resistance in cereals [7]; nevertheless, very dormant seed is regarded as to become a limiting issue in acquiring uniform germination and early seedling establishment [62]. As a result, to meet the contradictory demands of PHS resistance and proper germination when required, breeding programs need to incorporate alternate mechanisms into contemporary cultivars [2] like moderate dormancy in combination with modified spike morphology. AAC Tenacious can be a extremely PHS resistant, tall, photoperiod-sensitive and red-grained Canadian wheat KDM5 list cultivar [68]. In addition, it possesses a gibberellic acid (GA)-sensitive tall plant height allele Rht-B1a and the brassinosteroidsensitive tall plant height allele Rht8a [74] at Xgwm261 locus [75]. Above attributes make AAC Tenacious assessment crucial, not just for red-grain connected factors, but also for alternate physiological mechanisms which includes photoperiodic response. To this objective, AAC Tenacious was crossed with all the white-grained, semi-dwarf, soft-textured and photoperiod-insensitiveTable 2 Particulars of previously identified pre-harvest sprouting resistance quantitative trait loci (QTLs) and candidate genesPreviously identified QTL(s)c QTL(s)/marker(s) None QPhs.ccsu-1A.1 Xbarc145 QPhs.ccsu-2A.three Qphs.hwwg-2A.1 107,349,82212,188,108 105,533,85718,803,206 35,052,2954,499,689 Qphs.sau-2D None QPhs.pseru-3A/TaPHS1 QPhs.ocs-3A.1, QDor-3A, MFT-3A Qphs.hwwg-3A.1 wsnp_Ex_rep_c67702_66370241, wsnp_Ra_ c2339_4506620, Xbarc57.two 17,351,80627,206,323 QPhs.ocs-3A.1, QPhs.ocs-3A.two QPhs.ccsu-3A.1 858,443,086,589 QGi.crc-3B None QPhs.cnl-3D.1 QGi.crc-3D Xbarc376 566,481,13398,343,827 QPhs.inra-3D QGi.crc-3D TaMyb10-D1 56,469,95643,554,202 Phs1 Phs1 QPhs.ocs-4A.1, QDor-4A; Sprouting QTL 439,276,91169,339,659 QPhs.ocs-4B.1 None Qphs.hwwg-5A.1 None None QFn.crc-7D 398,807,98656,267,808 160,103,13199,974,559 556,976,25458,357,114 620,094,24839,730,768 46,061,67102,506,349 AM panel OS21 Leader Zenkoujikomugi Opata Chinese Spring Danby AC D2 Receptor Formulation Domain AC Domain Renan AM panel AC Domain Cayuga USA Canada Other people France Canada Others Japan Canada Japan Mexico China USA Canada 774,475,70375,489,185 4,376,76929,546,644 AC Domain Canada [72] [71] [70] [34] [71] [70] [51] [52] [57, 59] [61] [57] [12] [73] SPR8198 India [58] [71] Zenkoujikomugi Japan [60] AM panel Others [70] Danby USA [12] T NI NS NI TI T TI NI T T NI T T NS T NS TI TI None None None None None None None TaMyb10-D1 MFT-3B-1 None AGO802D, HUB1, TaVp1-D1 4,443,008 1

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