Chizochytrium sp. by GC-MS. No 1 2 three 4 5 six 7 8 9 ten 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Retention time min (imply SD) 12.377 0.009 12.484 12.789 12.803 12.837 0.009 13.230 0.006 13.287 0.012 14.145 0.012 14.260 14.396 0.053 14.412 14.590 14.812 0.066 14.875 0.024 14.885 15.128 15.609 15.632 15.672 15.924 0.055 16.316 0.089 16.330 16.378 0.046 16.388 16.403 0.072 16.629 0.036 17.071 0.026 17.193 0.053 17.303 0.044 17.311 0.044 17.427 17.607 0.037 17.702 0.044 17.735 17.735 0.179 17.904 18.413 18.416 18.459 0.046 18.514 18.533 18.764 18.838 18.870 18.954 19.038 19.206 19.775 20.024 Compound (structure) Tridecane (1; n = 10) 2H-Pyran-2-one, tetrahydro-4-hydroxy-4-methyl- (17) Cyclohexanol, 2-methyl-5-(1-methylethyl)-, (1a, 2b, 5b)- (19) 4-Hydroxy-3,4,6-trimethylhept-5-enoic acid lactone (20) Cyclohexanol, 4-methyl-1-(1-methylethyl)- (18) 1-Pentadecene (5; n = 12) Tetradecane (1; n = 11) Pentadecane (1; n = 12) Trinonanoin (12; n = 7) Dodecanoic acid, BRaf manufacturer methyl ester (four; n = 10) Undecanoic acid, 10-methyl-, methyl ester (11; n = eight) Stearic acid, 1,two,Adenosine Receptor Purity & Documentation 3-propanetriyl ester (12; n = 16) Tetradecanoic acid (three; n = 12) 1-Hexadecene (5; n = 13) Dodecanoic acid, ethyl ester (6; n = ten) Tridecanoic acid, methyl ester (four; n = 11) 9-Hexadecenoic acid, tetradecyl ester, (Z)- (16; n = 13) Tridecanoic acid, ethyl ester (six; n = 11) Stigmasterol (23) Methyl tetradecanoate (4; n = 12) Hexadecanoic acid (3; n = 14) 1-Eicosanol (2) Tetradecanoic acid, ethyl ester (6; n = 12) 1-Octadecene (5; n = 15) 7-Tetradecenoic acid, (Z)- (7; n = five, m = 5) Pentadecanoic acid, methyl ester (4; n = 13) Pentadecanoic acid, ethyl ester (six; n = 13) 9-Hexadecenoic acid, methyl ester, (Z)- (8; n = 5, m = 7) Hexadecanoic acid, methyl ester (4; n = 14) Pentadecanoic acid, 14-methyl-, methyl ester (11; n = 12) Germanicol (24) Ethyl 9-hexadecenoate (9; n = 5, m = 7) Hexadecanoic acid, ethyl ester (6; n = 14) Octadecanoic acid, 2-hydroxy-1,3-propanediyl ester (13) Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester (14) Heptadecanoic acid, methyl ester (4; n = 15) 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (ten) 9-Octadecenoic acid, methyl ester (8; n = 7, m = 7) 9-Octadecenoic acid (Z)-, 2-hydroxy-1-(hydroxymethyl)ethyl ester (15) 11-Octadecenoic acid, methyl ester, (Z)- (eight; n = five, m = 9) Octadecanoic acid, methyl ester (4; n = 16) 9-Hexadecenoic acid, eicosyl ester, (Z)- (16; n = 19) Ethyl 9-octadecenoate (Z)- (9; n = 7, m = 7) Cholesterol (22) Octadecanoic acid, ethyl ester (6; n = 16) 1-Dodecanol, 3,7,11-trimethyl- (21) Docosanoic acid, 1,2,3-propanetriyl ester (12; n = 20) Erucic acid (7; n = 7, m = 11) Eicosanoic acid, methyl ester (4; n = 18)Mioso et al.Figure 1 – Lipid compounds identified in Schizochytrium sp.sterols (22, 23), triterpenes (24), mono- and sesquiterpenes (17-21). Occurrence of branched fatty acid methyl esters The methyl-branched fatty acids are extensively distributed within the nature (Carballeira et al., 2001; Nechev et al., 2002). Now is known, that they are formed by the selective incorporation of your methylmalonyl-CoA, catalysed by the fatty acid synthetase enzyme (Seyama et al., 1981), and that such bioenergetic pathway is characteristic from bacteria that make relative higher concentrations of those isomethylbranched fatty acids. That are, as a result, accepted as molecular markers of your organic matter created by bacteria (Kaneda, 1991; Zabeti et al., 2010). Hence, the i.
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