Viv circular dichroism spectrometer model 62DS. Chemical syntheses See Supplementary InformationViv circular dichroism spectrometer model

Viv circular dichroism spectrometer model 62DS. Chemical syntheses See Supplementary Information
Viv circular dichroism spectrometer model 62DS. Chemical syntheses See Supplementary Information and facts for full experimental details and procedures of all performed reactions on the syntheses of substrate analogs, also as their full characterization (1H and 13C nuclear magnetic resonance, high-resolution mass spectrometry, infrared, optical rotation, melting point, and Rf value). All reactions had been carried out under an inert nitrogen atmosphere with dry solvents under anhydrous conditions unless otherwise stated. Dry acetonitrile (MeCN), dichloromethane (DCM), diethyl ether (Et2O), tetrahydrofuran (THF), toluene (PhMe) and triethylamine (Et3N) had been obtained by passing the previously degassed solvents through activated alumina columns. Reagents were bought in the highest commercial good quality and made use of without additional purification, unless otherwise stated. Yields refer to chromatographically and spectroscopically (1H NMR) homogeneous material, unless otherwise stated. Reactions were monitored by thin layer chromatography (TLC) carried out on 0.25 mm E. Merck silica plates (60F-254), applying UV light because the visualizing agent and an acidic remedy of p-anisaldehyde and heat, ceric ammonium molybdate and heat, or KMnO4 and heat as developing agents.Nature. Author manuscript; available in PMC 2014 May 28.Teufel et al.PageFlash silica gel chromatography was performed making use of E. Merck silica gel (60, particle size 0.043.063 mm). IR experiments were recorded on a Perkin-Elmer Spectrum one hundred FT-IR spectrometer. Melting points were recorded on a Fisher-Johns 12-144 melting point apparatus and are uncorrected.Author Manuscript Author Manuscript Author Manuscript Author ManuscriptSupplementary MaterialRefer to Internet version on PubMed Central for supplementary material.AcknowledgementsThis investigation was supported by US National Institutes of Wellness (NIH) grant R01AI47818 to B.S.M., NSF award nos. EEC-0813570 and MCB-0645794 plus the Howard Hughes Health-related Institute to J.P.N., NSF grant CHE-1213620 to B.P., and by fellowships to R.T. from the Deutsche Forschungsgemeinschaft (TE 931/1-1) and to A.M. from JSPS (21-644). We thank Marianne Bowman (Salk Institute) for technical help, Dr. Yongxuan Su (UCSD) for MS measurements, D-H. Huang and L. Pasternack (TSRI) for NMR spectroscopic help, A. Rheingold (UCSD) for X-ray crystallographic evaluation, and Christian Hertweck for establishing the synthesis of 26.
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