Product Name :
Rabbit anti-HER2 Polyclonal Antibody
Synonym :
HER2; MLN19; NEU; NGL; CD340
Host :
Rabbit
Species Reactivity:
Human
Specificity :
HER2 Antibody detects endogenous levels of total HER2.
Predicted Reactivity:
Zebrafish, Chicken, Rabbit, Xenopus, Dog, Horse, Bovine, Sheep, Pig
Applications :
WB: 1:500-1:2000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500
Immunogen:
A synthesized peptide derived from Human ERBB2(Accession P04626), corresponding to amino acid residues R1230-V1255.
Concentration :
1mg/ml
Purification :
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality:
Monoclonal antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Background :
Receptor tyrosine-protein kinase erbB-2 is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B. The human protein is also frequently referred to as HER2 (human epidermal growth factor receptor 2) or CD340 (cluster of differentiation 340). HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. But contrary to other member of the ERBB family, HER2 does not directly bind ligand. HER2 activation results from heterodimerization with another ERBB member or by homodimerization when HER2 concentration are high, for instance in cancer. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.
UniProt :
P04626
Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. |Synonym HER2; MLN19; NEU; NGL; CD340 |Host Rabbit |Specificity HER2 Antibody detects endogenous levels of total HER2. |Species Reactivity Human |Predicted Reactivity Zebrafish, Chicken, Rabbit, Xenopus, Dog, Horse, Bovine, Sheep, Pig |Applications WB: 1:500-1:2000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500 |Immunogen A synthesized peptide derived from Human ERBB2(Accession P04626), corresponding to amino acid residues R1230-V1255. |Properties |Form liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal antibody |Clone 069-57 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Receptor tyrosine-protein kinase erbB-2 is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B. The human protein is also frequently referred to as HER2 (human epidermal growth factor receptor 2) or CD340 (cluster of differentiation 340). HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. But contrary to other member of the ERBB family, HER2 does not directly bind ligand. HER2 activation results from heterodimerization with another ERBB member or by homodimerization when HER2 concentration are high, for instance in cancer. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approximately 30% of breast cancer patients. |tissuespecificity Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. |Post-translational modifications Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). |Cellular localization Cytosol;Endosome;Nucleus;Plasma Membrane; |UniProt P04626 | Western blot analysis of HER2 expression in A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-ErbB2 Recombinant Monoclonal Antibody(069-57)
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
93839-89-5 Biological Activity 2229974-83-6 medchemexpress PMID:29381010 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
