We identified that the EMT regulator ZEB2 is one of direct target genes of miR-132

minor overlap with our data. This study is the first comprehensive work evaluating clinical data with respect to prognosis and gene expression as well as comparative in vitro analysis of multiple FLT3 mutations. Our results strengthen the evidence that FLT3 mutations PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19632594 have varying activating potential and reject the strict division into 1702259-66-2 driver and passenger mutations. Although not every single mutation might affect the prognosis and outcome of AML, all functionally characterized mutants showed a gain-of-function phenotype in vitro. Therefore FLT3 point mutations can contribute to leukemogenesis and are thus potential targets for therapeutic interventions especially with regard to tyrosine kinase inhibitor resistance. subsequently reprobed against total AKT and MAPK. Semi-quantitive analysis of AKT and MAPK band intensity was performed to calculate the ratio between pAKT and AKT as well as pMAPK and MAPK. Values are expressed as mean +/2 S.D. of three independent experiments. indicates significance to FLT3-WT expressing cells. The signal intensity of Ba/F3 MIY expressing cells has been subtracted from FLT3-WT and FLT3 mutant values. tions in AML patients. Differential gene expression analysis of FLT3-ITD and -TKD mutations with respect to NPM1 mutation status. Only genes significant at p0.05 after adjustment for multiple testing are displayed. mutation status using GSEA. GSEA analysis of FLT3-ITD and -TKD with FLT3-WT using the “c2kegg”gene sets. Only gene sets with FDR,25% are displayed. ES: enrichment score; NES: nominal enrichment score; NOM p-val: nominal p-value; FDR q-val: false discovery rate. The endocannabinoid system consists of receptors, endogenous ligands, and ligand metabolic enzymes. Metaphorically the eCB system represents a microcosm of psychoneuroimmunology or mind-body medicine. Cannabinoid receptor 1 is the most abundant G protein-coupled receptor expressed in the brain, with particularly dense expression in: the substantia nigra, globus pallidus, hippocampus, cerebral cortex, putamen, caudate, cerebellum, and amygdala. CB1 is also expressed in non-neuronal cells, such as adipocytes and hepatocytes, and in musculoskeletal tissues. Cannabinoid receptor 2 is principally associated with cells governing immune function, although it may also be expressed in the central nervous. The quintessential eCB ligands are N-arachidonylethanolamide and sn-2-arachidonoylglycerol. AEA and 2-AG are released upon demand from cell membrane- embedded phospholipid precursors. The primary biosynthetic enzyme of AEA is N-acyl-phosphatidylethanolamine phospholipase D. 2-AG is biosynthesized by two isoforms of diacylglycerol lipase, DAGLa and DAGLb. AEA and 2-AG work in a homeostatic fashion, thus they are broken down after they activate CB1 or CB2. AEA is catabolized primarily by fatty acid amide hydrolase 1, and 2-AG is catabolized by monoacylglycerol lipase, PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19631907 and, to a lesser extent, a,bhydrolase-6, cyclooxygenase 2, and FAAH1. This “classic eCB system”has expanded with the discovery of secondary receptors, ligands, and ligand metabolic enzymes. For example, AEA, 2-AG, N-arachidonoyl glycine and the phytocannabinoids D9-tetrahydrocannabinol and cannabidiol may also serve, to different extents, as ligands at GPR55, GPR18, GPR119, and several transient receptor potential ion channels. The effects of AEA and 2-AG can be enhanced by “entourage compounds”that inhibit their hydrolysis via substrate competition, 1 Systematic Review of eCB Modulatio