Recorded, rats were sacrificed, the P curve from the respiratory system constructed, and bronchoalveolar

Recorded, rats were sacrificed, the P curve from the respiratory system constructed, and bronchoalveolar lavage and aortic blood samples obtained. Outcomes Group H animals exhibited in comparison with group N animals a lower raise in peak inspiratory pressures (0.7 ?1.1 vs 2.four ?0.5 mmHg, P < 0.001), significant shift of the P curve to the left and lower total protein (113 ?42 vs 201 ?97 /ml, P = 0.047) and TNF (23.5 ?8.0 vs 35.2 ?8.5 pg/ml, P = 0,022) levels in BAL samples. Conclusion Moderate hypothermia attenuated lung injury during low PEEP, high FiO2 and moderate tidal volume ventilation in animals sensitized to injury by previous anesthesia and surgery. Acknowledgement Supported by the Research project MZO 00179906. Therefore, inhibition of nNOS/Nox4 may be an effective therapeutic target in patients with ALI.P19 Degradation of endothelial DNQX price glycocalyx provides new insights in the pathogenesis of septic shock microvascular failureR Nevi e1, R Favory2, X Marechal1 1School of Medicine, Lille, France; 2Calmette Hospital, Lille, France Critical Care 2007, 11(Suppl 2):P19 (doi: 10.1186/cc5179) Introduction Glycocalyx (GLX) is implicated in mechanotransduction of shear PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20799915 strain and microvascular blood flow. We tested whether GLX loss accounts for the microvascular dysfunction in sepsis and whether or not activated protein C (APC) preserves endothelial GLX integrity. Techniques Endotoxin LPS (10 mg/kg) was infused in rats treated or not with APC (240 /kg/hour). Adjustments in GLX were assessed by circulating levels of hyaluronan (a GLX constituent) and by GLXSCritical CareMarch 2007 Vol 11 Suppl27th International Symposium on Intensive Care and Emergency Medicineapparent thickness evaluated working with intravital microscopy by comparing 4 and 150 kDa dextran distribution as markers of GLX permeable and impermeable tracers, respectively. Intravital microscopy was employed to characterize mesentery functional capillary density. Because glycocalyx is really sensitive to free of charge radical, oxidative stress was evaluated by oxidation of dihydrorhodamine (DHR) in microvascular beds and by concentrations of heart malondialdehyde (MDA) and plasma carbonyl proteins (CP). Outcomes LPS elicited a four hours later profound reduction in GLX layer thickness and improve in plasma hyaluronan levels. LPS rats had decreases in capillary continuous flow, and substantial increases in intermittent and stopped flow capillaries compared with controls. The pressor responses to norepinephrine were drastically reduced, indicative of vascular hyporeactivity. In vivo oxidation of DHR and levels of heart MDA and plasma CP have been all enhanced in LPStreated rats. Interestingly, in LPS rats, APC reduced plasma hyaluronan levels and GLX destruction, which was accompanied with important improvements in vasopressor response and functional capillary density. APC treatment also prevented increases in biochemical and in vivo microvascular oxidative strain markers. Conclusion In our model of septic shock, increased plasma hyluronan levels and reduction in endothelial layer thickness indicated GLX degradation. APC prevented vascular oxidative anxiety and limited GLX loss. GLX degradation plays a crucial role within the septic vasculature and generation of free of charge radicals through septic shock is potentially toxic to GLX function.P20 Exhaled breath condensate mediators in mechanically ventilated brain-injured patients with no acute lung injury are largely connected to markers of systemic inflammationI Korovesi1, E Papadomiche.