Older than 7 years and teens, is related with the development of asthma. The involvement

Older than 7 years and teens, is related with the development of asthma. The involvement of M. pneumoniae within the pathogenesis of respiratory diseases is as a result of the multiplicity of pathogenicity elements, the big of which can be hydrogen peroxide, which is released in enzymatic reaction catalyzed by glycerol-3-phosphate oxidase (genename MPN051). Within the structure on the enzyme amino acid His in position 51 is significant, due to the fact His51 participates in proton transfer during the oxidase reaction along with the price of this method determines the price of hydrogen peroxide formation. The aim from the study was to detect possible mutations in His51 codon containing fragment (location: from 50 to 260 in gene) of MPN051, capable to influence the rate of hydrogen peroxide formation in M. pneumoniae isolates. Approaches: Mycoplasma pneumoniae was isolated from sputum and throat swabs of 54 children and teens (77 years old) with pneumonia, 18 of them had also asthma, having a preliminary optimistic result of M. pneumoniae DNA detection by PCR. SMPT Description MPN051 gene of all M. pneumoniae isolates was tested for mutations by sequencing. Around the 10th day of culturing for all M. pneumoniae isolates the formation of hydrogen peroxide was studied in a semiquantitative peroxide test. Outcomes: Mutations related with decreased and enhanced levels of hydrogen peroxide production by M. pneumoniae have been detected. Mutation A152T, leading to change His51Leu, was observed in 7 isolates of M. pneumoniae from youngsters and teens with pneumonia with no asthma and was related with decreased production of hydrogen peroxide (about four mgl) in comparison with M. pneumoniae isolates without the need of mutations (about ten mgl). Mutation G163C, leading to alter Asp55His, was associated with enhanced production of hydrogen peroxide (about 20 mgl) and was prevalent in M. pneumoniae isolates from children and teens with pneumonia and asthma (61 ). The newly appeared His55 near His51 might promote proton transfer throughout the oxidase reaction, thereby accelerating the formation of hydrogen peroxide and enhancing the pathogenic properties of M. pneumoniae. Conclusions: Missense mutation G163C in MPN051 is connected with enhanced M. pneumoniae pathogenic properties and is prevalent in children and teens with mycoplasma associated pneumonia and asthma. P43 Does MrgprB3 plays human MrgprX2 function in rat mast cell Muhammad Novrizal Abdi Sahid, Shuang Liu, Takeshi Kiyoi, Kazutaka Maeyama 1 Ehime University, ToonShi, Japan Correspondence: Muhammad Novrizal Abdi Sahid rizal.pela@gmail. com Clinical Translational Benfluorex medchemexpress Allergy (CTA) 2018, 8(Suppl 1):P43 Background: Mast cells activation could occur through immunological and non-immunological pathways. In immunological pathway, the interaction among IgE and its receptor (FcRI) and the downstream signal rose from this interaction has been broadly studied and effectively characterized. However, the non-immunological pathway is less understood. The shade of light about this pathway begins by the report of Tatemoto et al. (2006) followed by McNeil and co-workers (2015), which states the existence of Mrgpr receptor family members that important within the non-immunological pathway in human and mouse, accordingly. Within the present report, we identify and characterized the Mrgpr receptor family members that responsible for the non-immunological activation of rat mast cells. Rat as long as mice are widespread animals that applied for the laboratory experiment inside the wide region of study. Therefore it really is vital.