Nal Allergy (CTA) 2018, 8(Suppl 1):P54 Background: Cross-reactive carbohydrate determinants (CCDs) are a

Nal Allergy (CTA) 2018, 8(Suppl 1):P54 Background: Cross-reactive carbohydrate determinants (CCDs) are a widespread trigger for elevated in vitro test outcomes, in particular when allergen sources of plant or insect venom origin are tested. This results in outcomes that will take a long time to interpret and clarify to sufferers. A new 4′-Methoxyflavonol Protocol multiplex diagnostic tool (Allergy Explorer ALEX) promises to block the binding of CCD specific IgE antibodies. Objective: We investigated the CCD IgE antibody blocking efficiency from the ALEX test system and its impact around the work of interpreting IgE testClin Transl Allergy 2018, 8(Suppl 1):Web page 22 ofresults. A additional target was to evaluate the performance from the two CCD markers (��)-Duloxetine Epigenetic Reader Domain spotted on this method (Ana c 2 and Hom s LF, human Lactoferrin–expressed in rice cells). Approaches: 9 CCD constructive samples (determined by ImmunoCAPo214, MUXF3) have been tested with ALEX prior to and after CCD blocking (performed with MUXF-HSA). Results: All samples showed optimistic results for Hom s LF and four out of nine samples had been discovered positive for Ana c 2 before CCD blocking. Immediately after blocking of CCD particular IgE antibodies the results for Hom s LF have been decreased by 100 in six out of 9 samples. The other three samples showed a blocking price of 822 . The Ana c 2 optimistic results were reduced to a single positive outcome, which was reduced by 65 . Inside a plethora of final results a one hundred CCD antibody blocking price was observed for grass pollen, certain tree pollen, weed pollen, cereals, fruit, legumes, mussels, nuts, seeds, spices, vegetables, hymenoptera venoms, latex and Ficus benjamina. Prior to blocking 446 optimistic final results have been obtained with ALEX. Following blocking 117 positive final results remained. Sera using a reduce MUXF3 value (two.0.0 kUAL) showed a CCD blocking efficiency (BE, determined by Hom s LF) of 8700 , one particular serum with 12.8 kUAL showed a 100 BE and 1 sample with 26.7 kUAL showed 82 BE. Conclusions: According to our outcomes Hom s LF is superior to Ana c 2 as a CCD marker, as 9 out of 9 samples were discovered optimistic compared to 4 out of 9 for Ana c 2. The dramatic decrease in good sIgE results (74 fewer optimistic results), in particular for pollen, foods, latex and hymenoptera venoms cause a shortened time for interpretation and explaining outcomes to patients. Patient’s anxieties more than good test results may also be lowered, particularly for high risk allergen sources. P55 Diagnostic value of optimistic outcome for recombinant allergen Api M 1 and Ves V 5 determined by IgE multiplex test ImmunoCAP ISAC: final results of multicenter study Urska Bidovec Stojkovic1, Martina Vachova2, Mira Silar1, Ziga Kosnik1, Mitja Kosnik1, Petr Panzner2, Peter Korosec1 1 University Clinic of Respiratory and Allergic Ailments, Golnik, Slovenia; two Department of Immunology and Allergology, Faculty of Medicine and Faculty Hospital in Plzen, Charles University in Prague, Prague, Czech Republic Correspondence: Urska Bidovec Stojkovic urska.bidovec@klinikagol nik.si Clinical Translational Allergy (CTA) 2018, 8(Suppl 1):P55 Background: Within the allergy field, the IgE multiplex test ImmunoCAP ISAC (ISAC) is an extra diagnostic tool for the assessment of complex circumstances. When employing the ISAC chip within a routine clinical laboratory setting optimistic outcome for recombinant allergen Api m 1 and Ves v five is regularly observed. In this multi-center study, we evaluated the meaning of this good outcome and its clinical relevance. Solutions: This study evaluated results of 2877 ISAC test for rVes v five andor rApi.