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Rabbit tooth for quantitative evaluation with the enamel-dentinal junction and the
Rabbit tooth for quantitative evaluation of the enamel-dentinal junction and the resin layer (Figure 3). TheDiagnostics 2021, 11,6 ofDiagnostics 2021, 11, x FOR PEER Overview three.2. IntensityProfile of OCT Images7 ofAn image intensity profile was extracted from the OCT image from the rabbit tooth for quantitative evaluation in the enamel-dentinal junction plus the resin layer (Figure 3). The intensity in Step followed by Step five, Step half maximum OCT image intensity in Step four was the maximum, followed by Step 5, Step 3, Step 1, and Step 2.Figure three. The OCT image intensity with profile distance in Methods 1, two, three, four and five. Dashed line: the Figure 3. The OCT image intensity with profile distance in Measures 1, 2, three, 4 and five. Dashed line: the half maximum of every single step. The half maximum OCT image intensity in Step four was the maximum, Step two. followed by Step 5, Step three, Step 1, and Step 2.3.three. SM Photos, SEM Images, and Histology three.three. SM Images, SEM Images, and Histology For SM photos, the enamel around the surface on the teeth in Step 1 was smooth and shiny For SM pictures, the enamel around the surface from the teeth in Step 1 was smooth and shiny (Figure 4A). A Tianeptine sodium salt Autophagy sizable location of dense white cloud-like spots was observed on on the tooth sur(Figure 4A). A big region of dense white cloud-like spots was observed the tooth surface in Step two (Figure 4D). The surface reflectivity of Step 3 (Figure 4G) and Step four (Figure 4J) face in Step two (Figure 4D). The surface reflectivity of Step three (Figure 4G) and Step 4 (Figure was weak, and the dense white stripe Bomedemstat In Vivo structures had been observed. In Step five (Figure 4M), the 4J) was weak, and also the dense white stripe structures had been observed. In Step 5 (Figure 4M), surface colour was even, and no white spots had been observed. the surface colour was even, and no white spots had been observed.Diagnostics 2021, 11, x FOR PEER Evaluation Diagnostics 2021, 11,eight of 7 of 13Figure four. pictures of SM (A,D,G,J,M), SEM (B,E,H,K,N), and histology (C,F,I,L,O) of each step. Figure four. TheThe photos of SM (A,D,G,J,M), SEM (B,E,H,K,N), and histology (C,F,I,L,O) of every step. Step 1: the surface on the incisor clean; Step two: 37 phosphate etching agent was evenly applied Step 1: the surface from the incisor waswas clean; Step two: 37 phosphate etching agent was evenly applied to the the incisor 15 min, after which the the surface was clean and dried; Step 3: Icon-Etch was smeared to incisor for for 15 min, following which surface was clean and dried; Step three: Icon-Etch was smeared evenly around the the surfaceincisor for 30 s, just after which the the surface was clean and dried; Step 4: Iconevenly on surface of of incisor for 30 s, immediately after which surface was clean and dried; Step four: IconDryDry was smeared evenly the the surfaceincisor andand kept for 1 min, after which the surface was was smeared evenly on on surface of of incisor kept for 1 min, after which the surface was dried; Step 5: Icon-Infiltrant was smeared evenly around the surface of maxillary incisor for 1 min and dried; Step 5: Icon-Infiltrant was smeared evenly around the surface of maxillary incisor for 1 min and light-cured for 40 s. Scale bar of SM is 500 m; scale bar of SEM is 25 m; scale bar of histology is 25 light-cured for 40 s. Scale bar of SM is 500 ; scale bar of SEM is 25 ; scale bar of histology is m. 25 .The second column of Figure four showed the SEM images of every single step. As shown in the second column of Figure 4 showed the SEM photos of each step. As shown Figure 4B, the surface in Step 1 was flat and dense, and no cracks were fou.

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