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Jiang et al. Journal of Experimental Clinical Cancer Research (2017) 36:131 DOI ten.1186/s13046-017-0602-RESEARCHOpen AccessmiR-19b-3p promotes colon cancer proliferation and oxaliplatin-based chemoresistance by targeting SMAD4: validation by bioinformatics and experimental analysesTao Jiang1, Ling Ye2, Zhongbo Han3, Yuan Liu2, Yinxue Yang1, Zhihai Peng2 and Junwei Fan2AbstractBackground: As a illness with exceptionally complicated molecular mechanisms, a lot of deregulated miRNAs have been identified in colon cancer. Handful of studies have already been performed by utilizing Ingenuity Pathways Analysis (IPA) to predict miRNAs especially expressed in colon cancer. Strategies: A characteristic microRNA-target network of colon cancer was explored applying IPA. Then the clinical significance of miR-19b-3p was evaluated in 211 colon cancer individuals.Annexin A2/ANXA2, Human The roles of miR-19b-3p and its candidate target gene, SMAD4, in colon cancer progression were examined each in vitro and in vivo.VHL Protein manufacturer Results: Bioinformatics analysis showed that 15 microRNAs screened by IPA had been drastically correlated with malignant biological behaviors of colon cancer.PMID:24278086 miR-19b-3p was the most substantially upregulated candidate determined by the validation experiment making use of 211 colon cancer samples. Higher expression of miR-19b-3p was substantially associated with high N stage (P sirtuininhibitor 0.001), higher AJCC stage (P sirtuininhibitor 0.001), poor histologic grade (P = 0.032), frequent venous and lymphatic invasion (P = 0.027), and liver metastasis (P sirtuininhibitor 0.001). Survival analysis revealed that miR-19b-3p was an independent prognostic issue connected with colon cancer patient’s general survival (OS) and disease-free survival (DFS). miR-19b-3p promoted proliferation and chemoresistance of colon cancer cells, but had no impact on invasion in vitro, in conjunction with tumorigenesis in vivo. Furthermore, we confirmed that miR-19b-3p mediates resistance to oxaliplatin-based chemotherapy by means of SMAD4. Conclusions: Our findings demonstrate the part of miR-19b-3p-SMAD4 axis in colon cancer progression, which may well turn out to be a possible therapeutic target against chemotherapy resistance. Key phrases: miR-19b-3p, SMAD4, Proliferation, Oxaliplatin, Chemoresistance Correspondence: 18895007660@163; pengzhihai1958@163; drjunweifan@163 Equal contributors 1 Division of Anal-Colorectal Surgery, Common Hospital of Ningxia Health-related University, Yinchuan 750004, People’s Republic of China two Department of General Surgery, Shanghai Basic Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20080, People’s Republic of China Full list of author info is obtainable at the finish on the articlesirtuininhibitorThe Author(s). 2017 Open Access T.