Product Name :
Nile Blue A sulfate
Description:
Nile Blue A (Nile blue sulfate) is used to differentiate melanins and lipofuscins. It is also useful for staining fats and preparation of an amperometric glucose sensor.
CAS:
3625-57-8
Molecular Weight:
732.85
Formula:
C40H40N6O6S
Chemical Name:
bis(12-amino-N,N-diethyl-8H-10-oxa-5-azatetraphen-8-iminium) sulfate
Smiles :
CC[N+](CC)=C1C=C2OC3=CC(N)=C4C=CC=CC4=C3N=C2C=C1.CC[N+](CC)=C1C=C2OC3=CC(N)=C4C=CC=CC4=C3N=C2C=C1.[O-]S([O-])(=O)=O
InChiKey:
QIRDPEPUXNCOLD-UHFFFAOYSA-N
InChi :
InChI=1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.{{FMK} web|{FMK} MAPK/ERK Pathway|{FMK} Protocol|{FMK} In Vitro|{FMK} manufacturer|{FMK} Cancer}
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
Nile Blue A (Nile blue sulfate) is used to differentiate melanins and lipofuscins. It is also useful for staining fats and preparation of an amperometric glucose sensor.|Product information|CAS Number: 3625-57-8|Molecular Weight: 732.85|Formula: C40H40N6O6S|Chemical Name: bis(12-amino-N,N-diethyl-8H-10-oxa-5-azatetraphen-8-iminium) sulfate|Smiles: CC[N+](CC)=C1C=C2OC3=CC(N)=C4C=CC=CC4=C3N=C2C=C1.CC[N+](CC)=C1C=C2OC3=CC(N)=C4C=CC=CC4=C3N=C2C=C1.[O-]S([O-])(=O)=O|InChiKey: QIRDPEPUXNCOLD-UHFFFAOYSA-N|InChi: InChI=1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : ≥ 150 mg/mL (409.{{Flurbiprofen} site|{Flurbiprofen} COX|{Flurbiprofen} Technical Information|{Flurbiprofen} Description|{Flurbiprofen} supplier|{Flurbiprofen} Autophagy} 37 mM).PMID:24670464 H2O : Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥12 months if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule.|Products are for research use only. Not for human use.|
