Rabbit anti-Elk1 Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. |Synonym ELK 1; Elk1; ELK1 member of ETS oncogene family; ELK1 protein; ELK1; ETS transcription factor; ELK1_HUMAN; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene |Host Rabbit |Specificity Elk1 Antibody detects endogenous levels of total Elk1. |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Xenopus, Rabbit, Pig, Dog, Sheep, Bovine, Horse |Applications WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human ELK1(Accession P19419), corresponding to amino acid residues H377-Q396. |Properties |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Polyclonal Antibody |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity). |tissuespecificity Lung and testis. |Post-translational modifications Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity). |Cellular localization Mitochondrion;Nucleus; |UniProt P19419 | Western blot analysis of Elk1 expression in heatshock treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.