The homogenization buffer contained (in mM): 10 Tris-HCl, 5 NaF, one Na3VO4, 1 EDTA, 1 EGTA, 320 sucrose, protease inhibitor EDTA totally free pill (Roche Diagnostics, Germany) at pH seven.four
Measurement of soon after-hyperpolarization. An after-hyperpolarization of the membrane was induced by stimulating neurons...