According to the effectiveness of transfection, we chose CLIC4-siRNA (Figure S1A and S1B). CLIC4 siRNA transfection inhibited the expression of CLIC4 protein substantially in U251 cells following 24 h (Fig. 4A and 4B)
Figure 1. Hunger-induced autophagy in human glioma U251 cells. (A) Western blot examination of...