The dissociation reactions in presence and absence of HLA-DM had been began by adding to one particular tube dissociation mix having ten mM unlabeled HA peptide and 1 mM HLA-DM and towards the second tube only ten mM unlabeled HA peptide within a final volume of two mL
ipore, Massachusetts, USA). DR4 was purified by immunoaffinity with LB3.1-conjugated protein A column, as...